Amphotericin B Binds Ergosterol Instead of Forming Ion Channels
نویسندگان
چکیده
منابع مشابه
Lipid and stress dependence of amphotericin B ion selective channels in sterol-free membranes.
The idea that amphotericin B (AmB) may not require sterols to form ion selective channels has recently been criticized on the grounds that egg phospholipids commonly used in experiments may contain small amounts of sterol which associate with AmB to form AmB/sterol pore channel structures. It was recently shown in this laboratory that modest osmotic stress can enhance the formation of AmB chann...
متن کاملSynthesis-enabled functional group deletions reveal key underpinnings of amphotericin B ion channel and antifungal activities.
Amphotericin B is the archetype for small molecules that form transmembrane ion channels. However, despite extensive study for more than five decades, even the most basic features of this channel structure and its contributions to the antifungal activities of this natural product have remained unclear. We herein report that a powerful series of functional group-deficient probes have revealed ma...
متن کاملSHEET ELECTROMAGNETIC FORMING USING CONVEX PUNCH INSTEAD OF CONVAVE DIE
High speed and absence of a precise control over pressure distribution confine sheet Electromagnetic Forming into a die to simple shapes having shallow depth. It is possible to reach a higher depth by using a convex punch instead of a concave die. In this study, sheet Electromagnetic Forming on a punch and sheet Electromagnetic Forming into a die are investigated. The electromagnetic part of th...
متن کاملLarge-conductance cholesterol-amphotericin B channels in reconstituted lipid bilayers.
The antimycotic activity of amphotericin B (AmB) depends on its ability to make complexes sterols to form ion channels that cause membrane leakage. To study this phenomenon, surface pressure (pi) as a function of surface area (A) and pi-A hysteresis were measured in monolayers of AmB-cholesterol mixtures on the water-air interface. The most stable monolayers were produced from molecules of AmB ...
متن کاملAmphotericin primarily kills yeast by simply binding ergosterol.
Amphotericin B (AmB) is a prototypical small molecule natural product that can form ion channels in living eukaryotic cells and has remained refractory to microbial resistance despite extensive clinical utilization in the treatment of life-threatening fungal infections for more than half a century. It is now widely accepted that AmB kills yeast primarily via channel-mediated membrane permeabili...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microbe Magazine
سال: 2012
ISSN: 1558-7452,1558-7460
DOI: 10.1128/microbe.7.163.1